Science is apparently not for the easily-frustrated

Science is apparently not for the easily-frustrated Although most of the time I coast through my days in lab in a whirlwind of science glee, I have to admit that every once in a while, the lab doesnt love me back. Yesterday, for example, I was trying to get a little ahead by running a protein gel I had run the samples before and gotten a nice result, but my postdoc Albert asked me to run the samples in a slightly different permutation in order to get a nice figure for our paper. Since I had some free time yesterday, I thought Id run the gel and get it over with. The gel ran for about two hours, then I set it up to transfer the proteins to a nitrocellulose membrane (which binds proteins and is much more hardy than the acrylamide gel). As you might notice, a successful transfer requires the frazzled lab monkey to correctly orient several components the gel, the membrane, the electrodes on the gel box and yesterday, the complexity apparently got the best of me. When I checked the protein after the transfer, it wasnt there. Probably it ran away in the other direction shouting Freeeedommmmm. I dont know. It was pretty frustrating to realize I had wasted an entire day. That, of course, doesnt really hold a candle to something I did this summer I was boiling some protein samples in a beaker with water in the bottom, and all the water boiled off. The beaker heated up and cracked, causing the plastic sample tubes to melt (and, obviously, causing the immunoprecipitation samples in them to become completely worthless). I got so flustered about having lost five days of work that I forgot about the DNA gel I was running; the samples ran off into oblivion. That was an ultra-trainwreck day. One day my sophomore year, I was working with the mice in the basement of the biology building. We have different mouse genotypes, and to tell them apart we have to take a small piece of their tails (dont worry, they dont mind), extract DNA from it, and run PCR. I take the tail sample with a straight razor blade. So one thing you should know about three-week-old mice is that they can jump really high. And they are really skittish around people. So naturally, as a sophomore new to my job, I was a little skittish around them. On this particular day, one of the mice had been wriggling around viciously, and I thought he was going to get loose. I was paying more attention to the mouse than to the razor blade and sliced my finger open with the razor blade. To add insult to injury, I didnt know where the band-aids were (I was new! and scared!), and didnt want to go back to the lab without having tailed the rest of the mice. So I wrapped my finger in some gauze, double-gloved, and tailed the rest of the mice. Hmm, what else one day I was doing a DNA extraction using phenol chloroform, which is a really nasty organic solvent. As you can see, the material safety data sheet strongly advises you to freak out if you spill it on yourself. I spilled some and decided to see if it really was as bad as the data sheet suggested. Um, yeah. It was. Trust the material safety data sheet. It doesnt lie. (Although phenol chloroform gives a really cool burn it turns your skin all white and bubbly so it was totally worth the experiment. But damn, it hurt.) On to questions! 1. s, who is now the official Queen of the Nerds (woot), asked why I didnt do the 5-year bachelors/masters program. Actually, that program (its called MEng) is only available for certain engineering majors, most notably course 6 (EECS) biology doesnt offer one. (For that matter, I dont even think the biology department at MIT offers a masters degree to anyone masters degrees arent really all that useful or common in the pure sciences. Check out the registrars data.) 2. Anna asked if it was possible to defer enrollment at MIT. According to this site, Occasionally, students may wish to take a year off between secondary school and college. In such cases, it is recommended that the student follow normal admissions procedures, as if going directly on to college, and then request deferment. Deferrals are granted for any reason except full-time enrollment at another university. As for whether youd get rusty on school stuff, you might shoot that question to Anthony, who took two(?) years off between high school and MIT. 3. (A question from a while back that I forgot to answer last time.) Mohan asked if writing the application which major/department are you psyched about essay on a less common major will help your chances of getting into MIT. Nope! The brilliant and wonderful readers will just use that answer as a way to gauge what youre excited about at MIT. MIT doesnt admit students by major, and youre totally free (once you get to MIT) to major in something entirely different from what you wrote down on the application. Writing that you want to major in something common wont hurt you, and writing that you want to major in something rare wont help you. 4. Anonymous asked what my GPA and GRE scores were. (For the record, I do not think these two numbers got me into graduate school. PhD programs in the sciences are all about your research experience and recommendations. Nonetheless.) My cumulative GPA when I applied was a 3.44 (on a four-point scale). On the GRE, I got an 800 (92nd percentile) in math and a 740 (99th percentile) in verbal. I got an 870 (97th percentile) on the biology subject test. 5. Christina asked why I said Complications reminded me why Im not premed. It had nothing to do with the description of life as a doctor it just got a little graphic at times, and blood and gore makes me sick to my stomach, thats all. :) and as a final note, I will mention that I may not be a premed, and I may not have straight As but I did get into Harvard Medical School! :D Thanks, everyone, for your congratulations Im still walking on air. So everybody who ends up at MIT next year, Ill still be around! EDIT: (for people who didnt follow the link!) No, Im not going to medical school the Harvard PhD program to which I was accepted is administered through the medical school, but its a PhD program, not an MD program. And I havent decided yet where Im going I still have five interviews in front of me!